Freddie Mercury (born Farrokh Bulsara; 5 September 1946 – 24 November 1991) was a British singer, songwriter, record producer, and lead vocalist of the rock band Queen. He died within 24 hours of confirming publicly that he had AIDS.
In "McStroke", Peter rejects Brian's implication that his new mustache is gay by noting that Freddie Mercury had one, describing it as proof of his heterosexuality.
Stewie becomes terrified of Queen's News of the World album in "Killer Queen". Brian alludes to Freddie's death by noting that most of the band is fine.
When he tags along for Brian's cutaway gag in "Connie's Celica", Stewie imagines himself with a Freddie Mercury sleeve tattoo.
In "Tales of Former Sports Glory", Quagmire tells of being a tennis prodigy, and getting to play in England in front of a queen after winning the US Open. Said 'queen' is Freddie Mercury.