Looney Tunes is a Warner Bros. animated cartoon series which ran in numerous movie theaters from 1930 to 1969. It preceded the Merrie Melodies series and is Warner Brothers's first animated theatrical series. The regular Warner Bros. animation cast also became known as the Looney Tunes. The name Looney Tunes is a variation on Silly Symphonies, the name of the Walt Disney Company's concurrent series of music-based cartoon shorts.
In "PTV", Coyote appears in a cutaway when Peter claims he worked for ACME. He offers a store credit when Wile E. claims a refund for a giant sling shot that slammed him into a mountain. Mrs. Coyote then appears telling her husband to hurry up.
Foghorn Leghorn visits a KFC in "Back to the Woods". He greets Colonel Sanders complimenting the smells of the spices. Sanders then decapitates him with a meat cleaver. Leghorn watches his headless body run around with guidance, not realizing his head was cut off until mid-sentence.
Rapid Dave is the American equivalent of Speedy Gonzales. He was created by Peter in "Padre de Familia" as a way of cleansing children's entertainment of foreign characters. Sylvester the Cat is seen chasing Dave before he escapes.
Luke Skywalker, played by Chris Griffin, has a typical Wile E. Coyote fall during his duel with Darth Vader, played by Stewie Griffin, in Something, Something, Something, Dark Side. He holds up a "HELP" sign, and then his body from the neck down droops before his whole body falls.
Peter and Mahmoud watch a Muslim version of Looney Tunes in "Turban Cowboy" where Porky Pig makes his traditional appearance though the drum head, although noting that under Islamic Law he is forbidden as dirty.
When Lois demands sex from someone when Peter is unable to perform in "Peter Problems", Peter cautions her that Quagmire will show up just like the Road Runner. Quagmire proceeds to do just that, including carrying a sign stating "Giggity Giggity." Peter reassures Lois that she has nothing to really fear, as her vagina is really just a painted on hole.
When Peter promises Lois to be gentile in his attempt to help Jesus lose his virginity in "The 2000-Year-Old Virgin", he claims he will be as gentile was the Tasmanian Devil tucking in his kids. A cutaway shows Taz reading a bedtime story. After he finishes, he kisses his child goodnight and starts to wind up his routine tornado exit, only to pause and gently slip from the room.
"Family Guy Lite" makes a reference to the Looney Tunes short, "Rabbit Seasoning". When Brian gets kicked by his horse a fourth time, his nose is sent spinning, similar to how Daffy Duck's beak turns when Elmer Fudd shoots him. Brian then moves his nose back to normal and uses the catchphrase, "You're despicable!"